Antioxidant Effect of Eugenol on Cytotoxic Activities of Retinoic Acid in Liver Tissue of NMRI Mouse

Purpose: To determine the inhibitory effects of eugenol on cytotoxic activities of retinoic acid in the liver tissue of the NMRI mouse. Materials and Methods: In this study, pregnant mice were administered by a single oral dose of 100 mg/kg eugenol from 5th to 10th days of pregnancy and also a single oral dose of 60 mg/kg RA at the day 10 of gestation. Pregnant females were sacrificed at the day 18 of gestation; the liver of the all the animals were studied by morphometric methods and examined for histological studies (H&E). Results: The results showed that eugenol had caused adjustment of the cell perimeter, cell area and polyploidy nuclear area of the liver cells, as well as reduction of nuclear area and maximum sinusoid diameter toward normal. Conclusion: The findings demonstrated that simultaneous administration of eugenol in certain doses caused the inhibition of retinoic acid activities, thereby enabling liver cells to remain in normal conditions.